Background Renewable energy production is currently a significant issue globally. decomposition of organic matter consist of people of the Eubacteria, class Clostridia, purchase Clostridiales, family members Clostridiaceae. Bacterias belonging in additional systematic groups donate to the diversity of the microbial consortium. Archaea comprise an amazingly little minority in this community, provided their crucial part in biogas creation. Among the Archaea, the predominant purchase may be the Methanomicrobiales and probably the most abundant species can be gene, which codes for just one of the main element enzymes in methanogenesis, the -subunit of methyl-coenzyme M reductase happening uniquely in methanogens . Alterations in the business of methanogenic communities under numerous conditions have already been reported based on this phylogenetic marker [15-19]. The automated Sanger sequencing strategy is frequently known as first era sequencing. Recent years possess brought important specialized breakthroughs and the next-generation sequencing methods have been created. A common feature of the methods, which use various chemical substance reactions for the fast dedication of DNA sequences [20,21], may be the creation of large databases ready from relatively brief sequence fragments and the usage of advanced bioinformatics to investigate the results . This metagenomic strategy enables the real-time research of live consortia in a variety of conditions through identification of the people of the communities [23-25] and/or dedication of the relative abundances of particular physiological features, reflected in the occurrence of particular enzymes [26-28]. The most widespread next-generation sequencing technique employs 454-pyrosequencing procedures for metagenomic purposes (Roche). This technique has been used for the characterization of biogas-producing communities [29-33], among numerous other applications. A fundamentally different methodology is offered by the SOLiD? (sequencing by oligo ligation and detection) technology (Applied Biosystems). As indicated by its name, SOLiD? Ciluprevir irreversible inhibition is based on a ligation reaction and each nucleotide is interrogated twice, which significantly Ciluprevir irreversible inhibition reduces the potential errors arising from misreading and thereby improves the reliability of the data [34,35]. Since its introduction onto the market in 2007, a number of systems have been investigated with the SOLiD? method [36-39], but as far as we are aware biogas-producing microbial communities have not been analyzed by SOLiD? so far. Besides its exceptional accuracy, the fundamental differences as compared with the 454-pyrosequencing approach are the extremely high throughput of the SOLiD system (200 Gb/run) and the short-read technology (50C75 nucleotides/read). The aim of the present study was to determine the possibility of applying this short-read next-generation sequencing technology to characterize the composite microbial consortium developing JMS in a biogas fermenter and to test whether the results validate those obtained by using the pyrosequencing approach. Samples were taken from an anaerobic fermenter fed primarily with plant biomass and pig manure slurry so that the conclusions could be compared with those drawn from other data sets relating to distinct anaerobic degradation processes with similar substrates. Results and discussion Distribution of metabolic functions in the microbial community In order to gain an insight into the diverse Ciluprevir irreversible inhibition biochemistry of the biogas-producing community, the short DNA sequences generated by Ciluprevir irreversible inhibition parallel sequencing were used to create environmental gene tags (EGTs) and clusters of orthologous groups of proteins (COGs). Ciluprevir irreversible inhibition The raw sequence reads of about 50?bp were assembled into contigs by using the CLC Bio Genomics Work Bench software . The generated contigs were uploaded to the MG-RAST server, where the data were automatically normalized, processed and evaluated. Those.