The phosphoprotein (P protein) of respiratory syncytial computer virus (RSV) is

The phosphoprotein (P protein) of respiratory syncytial computer virus (RSV) is a key component of the viral RNA-dependent RNA polymerase complex. SCR7 distributor recombinant viruses replicated well in Vero cells, rA2-PP2 and, to a greater extent, rA2-PP5, replicated poorly in HEp-2 cells. Virus budding from your infected HEp-2 cells was affected by dephosphorylation of P protein, because the majority of rA2-PP5 remained cell associated. In addition, rA2-PP5 was also more attenuated than rA2-PP2 in replication in the respiratory tracts of mice and cotton rats. Thus, our data suggest that although the major phosphorylation sites of RSV P protein are dispensable for computer virus replication in vitro, phosphorylation of P protein is required for efficient computer virus replication in vitro and in vivo. The phosphoprotein (P protein) of human respiratory syncytial computer virus (RSV), a prototype of the family D. M. Knipe and P. M. Howley (ed.), Fields virology, 4th ed., vol. 1. Lippincott Williams & Wilkins, Philadelphia, Pa. 6. Collins, P. L., M. G. Hill, E. Camargo, H. Grosfeld, R. M. Chanock, and B. R. Murphy. 1995. Production of infectious human respiratory syncytial computer virus from cloned cDNA confirms an essential role for the transcription elongation factor from your 5 proximal open reading frame of the M2 mRNA in gene expression and provides a capability for vaccine development. Proc. Natl. Acad. Sci. USA 92:11563-11567. [PMC free article] [PubMed] [Google Scholar] 7. Curran, J., R. Boeck, and D. Kolakofsky. 1991. The Sendai computer virus P gene expresses both an essential protein and an inhibitor of RNA synthesis by shuffling modules via mRNA editing. EMBO J. 10:3079-3085. [PMC free article] [PubMed] [Google Scholar] 8. Dupuy, L. C., S. Dobson, V. SCR7 distributor Bitko, and S. Barik. 1999. Casein kinase 2-mediated phosphorylation of respiratory syncytial computer virus phosphoprotein P is essential for the transcription elongation activity of the viral polymerase; phosphorylation by casein kinase 1 occurs mainly at Ser215 and is without effect. J. Virol. 73:8384-8392. [PMC free article] [PubMed] [Google Scholar] 9. Gao, Y., and J. Lenard. 1995. Multimerization and transcriptional activation of the phosphoprotein (P) of vesicular stomatitis computer virus by casein kinase-II. EMBO J. 14:1240-1247. [PMC free article] [PubMed] [Google Scholar] 10. Garcia-Barreno, B., T. Delgado, and J. A. Melero. 1996. Identification of protein regions involved in the interaction of SCR7 distributor human respiratory SCR7 distributor syncytial computer virus phosphoprotein and nucleoprotein: significance for nucleocapsid assembly and formation of cytoplasmic inclusions. J. Virol. 70:801-808. [PMC free article] [PubMed] [Google Scholar] 11. Gigant, B., F. Iseni, Y. Gaudin, M. Knossow, and D. Blondel. 2000. Neither phosphorylation nor the amino-terminal a part of rabies computer virus phosphoprotein TIL4 is required for its oligomerization. J. Gen. Virol. 81:1757-1761. [PubMed] [Google Scholar] 12. Grosfeld, H., M. G. Hill, and P. L. Collins. 1995. RNA replication by respiratory syncytial computer virus (RSV) is directed by the N, P, and L proteins; transcription also occurs under these conditions but requires RSV superinfection for efficient synthesis of full-length mRNA. J. Virol. 69:5677-5686. [PMC free article] [PubMed] [Google Scholar] 13. Hardy, R. W., and G. W. Wertz. 2000. SCR7 distributor The Cys3-His1 motif of the respiratory syncytial computer virus M2-1 protein is essential for protein function. J. Virol. 74:5880-5885. [PMC free article] [PubMed] [Google Scholar] 14. Horikami, S. M., J. Curran, D. Kolakofsky, and S. A. Moyer. 1992. Complexes of Sendai computer virus NP-P and P-L proteins are required for defective interfering particle genome replication in vitro. J. Virol. 66:4901-4908. [PMC free article] [PubMed] [Google Scholar] 15. Huber, M., R. Cattaneo, P. Spielhofer, C. Orvell, E. Norrby, M. Messerli, J. C. Perriard, and M. A. Billeter. 1991. Measles computer virus phosphoprotein retains the nucleocapsid protein in the cytoplasm. Virology 185:299-308. [PubMed] [Google Scholar] 16. Hwang, L. N., N. Englund, T. Das, A. K. Banerjee, and A. K. Pattnaik. 1999. Optimal replication activity of vesicular stomatitis computer virus RNA polymerase requires phosphorylation of a residue(s) at carboxy-terminal domain name II of its accessory subunit, phosphoprotein P. J. Virol. 73:5613-5620. [PMC free article] [PubMed] [Google Scholar] 17. Jin, H., X. Cheng, H. Z. Y. Zhou, S. Li, and A. Seddiqui. 2000. Respiratory syncytial computer virus that lacks open reading frame 2.