Aim of the study (and investigated its effects on the growth of the cells and possible synergy with anticancer drugs. two cell lines. Anti-cancer drugs alone induced growth inhibition, but there was no synergistic effect of extract on growth inhibition. Conclusions extract does not have a potent effect on the viability of cervical cancer cells extract has no synergistic effect on the inhibition of growth of cancer cells in the presence of anti-cancer drugs. (has been shown to lead to improvements in intestinal microbial balance [4], arthritis [5], and type 1 diabetes [6]. It has also been shown to have anti-cancer effects via growth inhibition in leukaemia [7] and liver cancer [8]. Many female cancer patients take as a nutritional supplement. Cervical cancer is the second leading cause of cancer mortality AB05831 IC50 among women worldwide. In Korea, in the past, cervical cancer had the highest incidence, but in recent years thyroid cancer and breast cancer have overtaken it. According to Korean Central Cancer Registry Project Statistics, cervical cancer accounted for one-eighth of all carcinomas in Korean women in 2009 [9]. The frequency of cervical cancer is gradually falling, largely because regular cancer screening prevents dysplasia and carcinoma from progressing to invasive cancer. However, cervical cancers occupy an important position among all cancers if carcinoma and dysplasia are included. Cervical cancer is associated with viral infection [10]; the most important cause of cervical cancer is persistent cervical infection with human papillomavirus AB05831 IC50 (HPV) [11]. Bacterial vaginosis (BV) is caused by an alteration in the vaginal flora involving a decrease in and predominance of anaerobic bacteria. A meta-analysis has shown that BV is associated with uterine cervical HPV infection [12]. Development of vaccines for the prevention and treatment of cervical cancer is ongoing. Traditional treatment methods for women’s cancer are surgery, radiation and chemotherapy. Surgery is carried out in cases with no metastasis to other organs, whereas chemotherapy and radiation are performed after surgery or in cases where there is metastasis to other organs. Many female patients take nutritional supplements, such as anti-oxidant agents, fish oil, and extract on growth inhibition in cervical cancer cells. Material and methods Bacteria strains and growth conditions ATCC393 (American Type Culture Collection) was obtained from the Korean Culture Centre of Microorganisms (KCCM). The bacteria were grown in Lactobacillus Mann-Rogosa-Sharp (MRS) broth (Difco 0881) at 37C. The growth medium consisted of 10 g Difco proteose peptone No. 2, 10 g beef extract, 5 g yeast extract, 20 g glucose, 1 g sorbitan monooleate complex (Tween 80), 5 g ammonium acetate, 5 g sodium acetate, 2 g K2HPO4, 1 g MgSO4-7H2O, and 0.05 g MnSO4-4H2O. The pH of the growth medium was adjusted to 6.5 after mixing the components. Preparation and purification of cell-free extracts MGC33570 of AB05831 IC50 was grown in MRS medium for two days until the mid-exponential phase (A.600 = 0.5), then it was centrifuged and re-suspended in 500 l 1 phosphate-buffered saline (PBS) with two further washes, and disrupted on ice for 1 minute with a Sonic Dismembrator at 5 Watts. The extract was centrifuged and the supernatant filtered through a 0.2 m syringe filter. The protein concentration of the extract was assayed by the Bradford method, with bovine serum albumin (BSA) as the standard. Cell culture CaSki and HeLa cell lines were obtained from the Korean Cell Line Bank (Seoul, South Korea). The cells were cultured on tissue culture dishes (Falcon; San Jose, CA, USA) in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% foetal bovine serum (FBS). Aliquots of 5 105 cells/dish were cultured at 37C in a humidified atmosphere containing 5% CO2. Cell growth rate measurement For each cell line, 5 105 cells were seeded in DMEM containing 10% FBS. After 24 hours the cells were washed with PBS and cultured in fresh medium. Cells were treated with distilled water, followed by 100 ng, 1 g, or 10 g/ml extract alone, anti-cancer drugs alone (doxorubicin 10 nM, 5-FU 10 nM, paclitaxel 10 nM, cisplatin 10 nM), or extract (10 g/ml) plus anti-cancer drugs (doxorubicin 10 nM, 5-FU 10 nM, paclitaxel 10 nM, cisplatin 10 nM) for 72 hours. The growth rate was determined using an inverted microscope (Olympus Model IX51, Tokyo, Japan) equipped with a DP50 camera system (Olympus, Tokyo, Japan) using the 0.4% trypan blue dye exclusion method. Cell survival was measured as a percentage of the total number of cells (viable plus non-viable). Mean survival was determined by counting four randomly selected non-overlapping fields. Each culture dish represents one determination, with each experiment replicated independently 4C6 times using different cultures. Cell cycle analysis CaSki and HeLa cells were seeded at a density of 1 105 cells/ml in DMEM medium containing 10% FBS. After 24 hours they were incubated with distilled water, extract (10 g/ml), or extract (10 g/ml) plus anti-cancer drugs (doxorubicin 10 nM,.