Introduction Ischemic heart disease is a significant public medical condition in traditional western countries. performed three different remaining anterior descending artery (LAD) ligation at different ranges (5, 10 and 15 mm) with regards to the apex. Four weeks after the severe myocardial infarction, we correlated lab investigations (serology, ECG, cardiac ultrasound) with histopathological results. Results Heart strategy attained by rib osteotomy (rib III, IV and V) in the costo-sternal junction level on the proper part, maintains the integrity from the ribcage, and can be a part of respiratory motions and the pet model doesn’t need oro-tracheal intubation. Ligation of LAD at 15 mm from the apex was incompatible with life; ligation of LAD at 5 mm from CB-7598 the apex does not achieved transmural myocardial infarction and ligation of LAD at 10 mm from the apex achieved a transmural myocardial infarction of the left ventricle which also involved the distal part of the interventricular septum. Conclusion Ligation of LAD at 10 mm from the apex achieved a transmural myocardial infarction of the left ventricle, is in an easily accessible area from technical point of view, it is sufficiently expanded to induce hemodynamic effects that can be quantified with paraclinical examination and also it is compatible with the experimental animal life. If the heart is approached by rib III, IV and V osteotomy at the costo-sternal junction level on the right side combined with neuroleptic anaesthesia, the animal does not need assisted ventilation. Keywords: left anterior descending (LAD), CB-7598 acute myocardial infarction, chronic myocardial infarction, coronary artery ligation, oro-tracheal intubation Introduction The induction of an experimental chronic myocardial infarction sufficiently expanded to cause a hemodynamic alteration that can be quantified with paraclinical examination and also to be compatible with the experimental animal life is an essential first step in post myocardial infarction ischemic heart disease research. In this study we aimed to find an optimal place for a coronary artery ligation to induce an optimal chronic myocardial infarction and also develop a new surgical approach of the heart that would not require oro-tracheal intubation. The literature describes several methods to achieve acute coronary occlusion in the experimental animal: Open chest approaches and coronary artery ligation [1,2,3,4,5,6,7,8]; Circumferential placement of an ameroid constrictor [9]; Progressive stenosis using non-elastic Dacron tapes [10]; Cryoinfarction [11,12]; Electrical coronary thrombosis [13,14]; Selective embolization via interventional methods of one or more coronary arteries [15,16,17,18,19]. The laboratory rabbit is the experimental animal that perfectly combines the technique accessibility with cost in the induction of myocardial infarction. The rabbit coronary anatomy, although inconstant, largely respects human coronary artery anatomy. It has two main coronary arteries, right coronary artery and left coronary artery, both originating at the ascending aorta. Left coronary artery is usually the dominant artery and has three main branches: Left proximal atrial artery; Left anterior descending artery (LAD), which descends on the anterior interventricular sulcus to the apex. It has, a lot of the correct instances, a septal branch that irrigates the interventricular septum (continues to be also described instances where septal artery originated straight from the trunk from the remaining coronary artery); Circumflex artery which ends using the marginal artery CB-7598 that products blood left side from the heart. The Mouse monoclonal to MAPK10 proper coronary artery is in charge of the perfusion of the proper center; septal branches provide you with the interventricular septum [20,21,22,23,24]. Concerning the keeping the coronary artery ligation, it should be chosen so the myocardial infarction created matches these requirements: To maintain an easy to get at region, from a specialized perspective; To become sufficiently CB-7598 extended to stimulate a hemodynamic impact that may be quantified by paraclinical exam; To be appropriate for the experimental pet life. To response the first necessity we pick the remaining anterior descending artery (LAD) to become ligated, a branch from the remaining coronary artery, in charge of vascularization of anterolateral and anterior area from the remaining ventricle, component and apex of interventricular septum [20]. Concerning the 3rd and second requirements, to be able to determine the perfect area of LAD ligation, we divided the band of rabbits into 3 subgroups and noticed three specific LAD ligatures at different ranges through the apex. Components and Methods As laboratory CB-7598 animals we used a group of 24 chinchilla.