Chromatin immunoprecipitation (ChIP) is a powerful method to determine protein binding to chromatin DNA. filtration procedure to obtain high-quality nuclei devoid of significant myofibril contamination. We prepared chromatin through the use of an ultrasonicator eventually, and ChIP assays with anti-BMAL1 MLN4924 manufacturer antibody revealed sturdy circadian binding pattern of BMAL1 to focus on gene promoters.… Continue reading Chromatin immunoprecipitation (ChIP) is a powerful method to determine protein binding