ABCB4 which is specifically expressed within the canalicular membrane of hepatocytes exports phosphatidylcholine (PC) into bile. was removed and used as the detergent-soluble fraction. Precipitate (detergent-insoluble fraction) was resuspended by sonication in HEPES-buffered saline (protease inhibitor 0.15 M NaCl 25 mM HEPES pH 7.4) ATP (Adenosine-Triphosphate) containing 1% detergent. Plat OptiPrep gradient fractionation Harvested BHK… Continue reading ABCB4 which is specifically expressed within the canalicular membrane of hepatocytes