In this scholarly study, the potential cytotoxicity of four plant extracts originated from Cameroon: (XA), (IC), (EG) and (DP) were examined in vitro. of probable genetic toxicity by these extracts revealed no or minimum incidence of genetic toxicity. Therefore, the studied plant extracts Mmp13 are exhibiting potent anticancer activity based upon marked induction of tumor-cell death. (XA) which is a member of the family Annonaceae, is used as a spice in Western and Central Africa, as well as to treat bronchitis, headache and ulceration [5]. In addition to its anti-diabetic effect [6], anti-anaphylactic and anti-inflammatory activities [7], several studies have shown that XA extracts possess antibacterial and antifungal activities [8,9,10,11]. (IC) (family Poaceae) also known as spear grass in West Africa, has diuretic, MK-4256 anti- inflammatory and antibacterial activities [12,13]. It also shows a potent anthelmintic activity [14] and the methanolic extract of its rhizomes was reported as a significant neuroprotective against glutamate-induced neurotoxicity in primary cultures of rat cortical cells [15]. (EG), (family Compositae) is traditionally used as a medicinal agent mainly in Africa and Asia. It is mainly used as heart and gastric troubles spice, reducing as well asthma attacks. In previous studies, the root methanolic extract showed significant antioxidant [16], antibacterial [17], and antifungal effects [18]. The methanolic extract of EG roots also exhibited a significant activity against M. tuberculosis [19], the methanolic extract from the underground part also reported for cytotoxic activity against prostate cancer (Mia PaCa2) and two leukemia cells (CCRF-CEM and CEM/ADR5000) [20]. (DP), (family Moraceae) is widely used in traditional medicine and represents a great source of active constituents including flavonoids, alkaloids and phenolic compounds [21,22,23]. It includes a therapeutic influence on cardiovascular disorders, snakebites, stomach and headache disorders, furthermore it displays a powerful antimicrobial activity so that as its methanolic demonstrated antibacterial activity against a -panel of Gram-negative bacterias including multidrug resistant (MDR) MK-4256 phenotypes [13]. Latest research reported the isolation of two isoprenylated flavones from the main draw out of DP that activate AMP-activated proteins kinase (AMPK), stimulate blood sugar uptake and lower glycemia [24]. Lately, various studies had been conducted for testing the cytotoxic activity of the plant components against a number of tumor types and level of resistance. These studies show a promising aftereffect of using these components against some tumor cell lines [25,26,27,28,29]. Regardless of the useful natural activity indicated by certain vegetation, the analysis of their possible toxicity remains important particularly. As some chemical substances or supplementary metabolites from vegetation are poisons like chemicals that could cause dangerous effects to human beings. In this scholarly study, a well-established evaluation from the anticancer potential of the four plant extracts: (i) XA, (ii) IC, (iii) EG and (iv) DP was performed to assess their activities in the inhibition of cell proliferation in seven different human cancer cell lines: HeLa (cervical), MDA-MB-231 (breast), A549 (lung), HepG2 (liver), U-87 (glioblastoma, brain), SK-OV-3 (ovarian) and HL60 (leukemia). As well, an assessment of in vitro toxicity of these plant extracts was performed in non-cancerous HEK-293 cells. HeLa cells showed a higher sensitivity in cell proliferation assay upon treatment with these extracts, so further studies of the alteration and induction of cell death were investigated in HeLa cell line, by comparing the treated cells with these extracts to the untreated cells, using different assays involving cell cycle analysis, the caspase 3/7 activity and mitochondrial membrane potential (MMP). The effect of the studied medicinal plants on MK-4256 the inhibition of cell progression and metastasis was assessed using the wound healing assay. Furthermore, a single cell gel electrophoresis assay was performed to exclude any probable genetic toxicity upon using of these extracts in MK-4256 cancer treatment. 2. Results and Discussion 2.1. Cell Proliferation Assay The anti-proliferation effect is the first indication to be assessed when investigation novel antitumor agents, thus the cell growth inhibitory activity of the four plant components was initially evaluated for the HeLa (cervical tumor) cell range 48 h after treatment with different concentrations from the crude methanol components. A dose reliant reduction in cell viability was noticed (Shape 1). At a focus of 50 g/mL of every crude draw out, EG and DP inhibited the cell development by even more.